Figure 4.

Luteolin attenuates rat bladder xenograft tumor growth through inactivation of mechanistic target of rapamycin (mTOR) and upregulation of p21. A, Tumor volume of BC31 xenografts in nude mice received a control diet or a diet with luteolin. B, H&E staining of BC31 tumors. C, Immunohistochemistry of Ki67, p21 and phospho(p)‐S6 in TUNEL assays of tumors. The bottom bar charts show quantitative levels. Mean ± SD, n = 10 (control), n = 9 (luteolin), *P < 0.05, **P < 0.01 and ***P < 0.001 are statistically significant compared with the control group