Figure 2.

MicroRNA (miR)‐92b‐3p mimic significantly upregulated cell growth, migration, and invasion activities in ACHN cells. A, Expression of miR‐92b‐3p in 4 renal cell carcinoma (RCC) cell lines was examined by quantitative real‐time PCR. B, ACHN cells transfected with miR‐92b‐3p mimic or negative control (NC) miRNA mimic for 24 h were reseeded in a 96‐well plate, incubated for the indicated times, and examined by MTS assay. Values are means ± SD of 3 independent experiments. *P < .05, **P < .01 vs. control mimic. C, ACHN cells were transfected with the miR‐92b‐3p mimic or a negative control miRNA mimic for 72 h. Cell migration was measured 18 h after a wound was created by scraping. Representative results of cell motility in the scratch wound‐healing assay are shown. Scale bar = 200 μm. Results are expressed as mean ± SD of 3 independent experiments. *P < .05 vs. control mimic. D, ACHN cells were transfected with the miR‐92b‐3p mimic or a NC miRNA mimic for 72 h. The transfected cell suspension was added to the upper chamber of Matrigel‐coated Transwell membrane inserts, and the lower chamber was filled with the complete medium and then cultured for 48 h. Invasive cells that had penetrated the Matrigel membrane were fixed and stained. Scale bar = 1.2 mm. Values are means ± SD of 3 independent experiments. *P < .05 vs. control mimic