Figure 4.

MicroRNA (miR)‐92b‐3p targets TSC1 in renal cell carcinoma (RCC) cells. A, Predicted miR‐92b‐3p‐binding site within the 3′‐UTR of the human TSC1 gene. B, ACHN cells were cotransfected with the luciferase reporter construct containing the predicted miR‐92b‐3p‐binding site within the TSC1 3′‐UTR and miR‐92b‐3p mimic, or a negative control (NC) miRNA mimic. C, Caki‐2 cells were cotransfected with the luciferase reporter construct containing the predicted miR‐92b‐binding site in the TSC1 3′‐UTR and miR‐92b inhibitor, or NC miRNA inhibitor. Relative luciferase activities were calculated as ratios of firefly luminescence / Renilla luminescence. Values presented as mean ± SD of 7 independent experiments (B,C). One‐way ANOVA with Tukey’s post hoc tests. *P < .05, **P < .01, ***P < .001