Figure 3.

Downregulation of anthrax toxin receptor 1 (ANTXR1) inhibits proliferation, migration, and invasion of gastric cancer (GC) cells. A, B, Knockdown of ANTXR1 was confirmed at the protein and mRNA level in BGC823 and MGC803 cells by western blotting and quantitative PCR. C, D, ANTXR1 knockdown inhibited proliferation of BGC823 and MGC803 cells as determined by CCK‐8 and colony formation assays. E, Transwell assays were undertaken to measure the effects of ANTXR1 downregulation on migration and invasion abilities of BGC823 and MGC803 cells. F, Western blot analysis of E‐cadherin, Vimentin, and snail levels in GC cells transfected with ANTXR1‐specific siRNA or control siRNA. *P < .05, **P < .01, ***P < .001. NC, negative control