Figure 4.

Overexpression of anthrax toxin receptor 1 (ANTXR1) promotes proliferation, migration, and invasion of gastric cancer (GC) cells. A, B, Overexpression (OE) of ANTXR1 was confirmed at the protein and mRNA level in HGC27 and SGC7901 cells by western blotting and quantitative PCR. C, D, Proliferation ability of ANTXR1 overexpression GC cells (HGC27/ANTXR1 and SGC7901/ANTXR1) without or with the PI3K inhibitor LY294002 (20 μM, 48 h) was determined by CCK‐8 and colony formation assays. E, Transwell assays were undertaken to measure the effects of ANTXR1 overexpression and LY294002 treatment on migration and invasion abilities of HGC27 and SGC7901 cells. F, Western blot analyses were used to measure the effects of ANTXR1 overexpression and LY294002 treatment on E‐cadherin, Vimentin, and snail levels. *P < .05, **P < .01, ***P < .001