Figure 5.
Anthrax toxin receptor 1 (ANTXR1) inhibits apoptosis of gastric cancer (GC) cells and accelerated cell cycle G1‐S transition in GC cells. A, B, Cell cycle distribution in different phases was analyzed by flow cytometry. C, D, Western blot analysis of cyclin D1, p21Cip1, and p27Kip1 in ANTXR1‐silenced cells, ANTXR1‐overexpressed (OE) cells, and ANTXR1‐overexpressed cells treated with LY294002. E, F, Cells were stained with annexin V‐Alexa Fluor 647/propidium iodide then apoptosis rates were assessed by flow cytometry. G, H, Western blot analysis of Bcl‐2, cleaved caspase 3, and Cytochrome C in ANTXR1‐silenced cells, ANTXR1‐overexpressed cells, and ANTXR1‐overexpressed cells treated with LY294002. *P < .05, **P < .01, ***P < .001