Figure 1.

High‐throughput screening methods for the identification of small inhibitors targeting DNA binding proteins. Left panel (quantitative protein interaction with DNA [QPID] assay): integrated microfluidic‐based assay with a DNA microarray to investigate the physical interactions of DNA binding proteins with their target sequences in high‐throughput screening. Middle panel (DNA‐protein interaction [DPI]‐ELISA): high‐throughput ELISA for identification of small molecules that inhibit the binding of DNA binding proteins to DNA sequences. Right panel (DNA strand exchange‐fluorescence resonance energy transfer [DSE‐FRET]): FRET‐based assay for identification of small compounds that inhibit the interaction of DNA binding proteins with DNA oligos, which leads to DNA strand exchange