Figure 6.

The combination of BKM‐120 and docetaxel, cisplatin and 5‐fluorouracil (TPF) chemotherapy potently inhibits the growth of oral squamous cell carcinoma (OSCC) xenografts. A, Bioluminescence imaging of tumor‐bearing mice. Representative images in four treatment groups are shown in the figure. B, Regions of interest from displayed images were revealed on the tumor sites and bioluminescence intensity (BLI) in tumors over time was measured, quantified as total radiance/s. C, Tumor‐bearing mice body weight in four treatment groups. D, Tumor samples were stained with H&E, and proliferating tumors cells were identified by immunohistochemistry for Ki67. Representative images from four groups are shown in the figure (original magnification × 200). E, Data shown represent mean ± SD of Ki67 index in of three random high‐power microscopic fields from tumors in each group. F, Tumor samples were harvested, lysed and analyzed by western blotting as indicated. *P < 0.05, **P < 0.01, n.s. = not significant. Data are expressed as mean ± SD