Figure 6.

Knockdown of p62 inhibits bladder cancer growth in vivo. A, 5637 cells were infected with single guide (sg) p62 and cultured for 48 h and then inoculated into nude mice. Tumor volume was calculated at indicated time points. B, C, Tumor appearance and weight of control and p62‐depleted 5637 cells are shown. D, Immunoblots of indicated proteins of tumors from control and p62‐depleted 5637 cell‐induced tumor. E, Representative immunohistochemistry images of nuclear factor‐E2‐related factor 2 (NRF2) and glutamate‐cysteine ligase catalytic subunit (GCLC). Scale bar, 50 μm. Data are shown as the mean ± SD, Student’s t test, *P < .05, **P < .01. Bcl‐2, B‐cell lymphoma‐2; Con, control; KEAP1, Kelch‐like ECH‐associated protein 1