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The conserved protruding nucleotide in L genome panhandles also plays a significant role in L UTR activity. The indicated wild type minigenome-luc plasmids based on viral L segments or the corresponding mutants with the protruding 10C deleted were transfected into BHK-21 cells. At 12 h post-transfection, cells were superinfected with SFTSV (MOI = 3), followed by luc activity measurements at 48 hpi. Data shown are mean ± SEM, n = 3. ∗∗P < 0.01; ∗∗∗P < 0.001.
