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. 2020 Apr 10;51:53. doi: 10.1186/s13567-020-00774-0

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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NDV and IL-1β expression levels in the organs of SPF chickens and DF1 cells.A NDV M gene expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. B IL-1β gene expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. C NDV-NP protein expression in the lungs, glandular stomach, and bursa of Fabricius in chickens. D IL-1β protein expression in the lungs, glandular stomach, and bursa of Fabricius of chickens. The lungs, glandular stomach, and bursa of Fabricius were collected at 3 dpi, and levels of NDV and IL-1β were determined by qPCR, Western blot, or ELISA. E NDV M gene expression in DF1 cells. F IL-1β gene expression in DF1 cells. G NDV-NP protein expression in DF1 cells. H IL-1β protein expression in DF1 cells. DF1 cells and cell culture supernatant were collected at the indicated time points, and the levels of NDV and IL-1β were determined by qPCR, Western blot, or ELISA. *P < 0.05, **P < 0.01, and ***P < 0.001 between the experimental and control groups.