Fig. 3.

Particle diffusion and brightness analysis by FCS and PCH analysis. (A) The diffusion coefficients of SMALP encapsulated ACBG2 and control proteins CD28 and CD86 were determined by 2-component fitting of the autocorrelation curve (Fig. 2B). The diffusion coefficients of the predominant component (C1; Table 2) in each case were not statistically different to each other. (B) The molecular brightness of the predominant component in PCH analysis of ABCG2 was equivalent to that of the dimeric control (CD28) and approximately double that of the monomer control (CD86). Data represent the mean ± SD of three independent experiments each consisting of 3 measurements and significance is shown following one way ANOVA and Sidak post-analysis.