Table 2.
SMALP diffusion coefficient as calculated by fluorescence correlation spectroscopy and subsequent autocorrelation analysis.
| Protein | Component 1 |
Component 2 |
||
|---|---|---|---|---|
| Fraction (%) | D (μm2s−1) | Fraction (%) | D (μm2s−1) | |
| ABCG2 | 88.5 ± 5.2 | 31.2 ± 4.3 | 11.5 ± 5.2 | 1.8 ± 0.5 |
| CD28 | 95.7 ± 2.7 | 32.5 ± 3.6 | 4.3 ± 2.7 | 2.8 ± 0.9 |
| CD86 | 98.7 ± 1.4 | 37.8 ± 3.0 | 1.3 ± 1.4 | 2.1 ± 0.9 |
FCS data were analysed with a 2-component model as described in the methods to determine diffusion components of SMALP particles in solution. Values given represent the mean ± SD for three independent experiments, with multiple fluctuation reads within each sample.