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. 2020 Jun 1;1862(6):183218. doi: 10.1016/j.bbamem.2020.183218

Table 2.

SMALP diffusion coefficient as calculated by fluorescence correlation spectroscopy and subsequent autocorrelation analysis.

Protein Component 1
Component 2
Fraction (%) D (μm2s−1) Fraction (%) D (μm2s−1)
ABCG2 88.5 ± 5.2 31.2 ± 4.3 11.5 ± 5.2 1.8 ± 0.5
CD28 95.7 ± 2.7 32.5 ± 3.6 4.3 ± 2.7 2.8 ± 0.9
CD86 98.7 ± 1.4 37.8 ± 3.0 1.3 ± 1.4 2.1 ± 0.9

FCS data were analysed with a 2-component model as described in the methods to determine diffusion components of SMALP particles in solution. Values given represent the mean ± SD for three independent experiments, with multiple fluctuation reads within each sample.