Table 3.

PCH analysis of SMALP encapsulated ABCG2, and monomeric (CD86) and dimeric (CD28) controls.

Protein	Component 1		Component 2
	Fraction (%)	ε (cpm.s−1)	Fraction (%)	ε (cpm.s−1)
ABCG2	94.6 ± 1.6	51,999 ± 2135	5.4 ± 1.6	408,163 ± 12,498
CD28	93.8 ± 1.3	60,030 ± 7406	6.2 ± 1.3	465,789 ± 71,770
CD86	93.0 ± 1.5	27,074 ± 6975	7.0 ± 1.5	271,957 ± 47,354

Fluorescence fluctuations were analysed by PCH analysis using a 2-component model as described in the text. Values given represent the mean ± SD for three independent experiments.