Fig. 7.

Comparison of the phospholipid profiles of bulk membrane lipids, and SMALP-extracted lipids, from P. pastoris.

Different classes of phospholipids were separated by HILIC and analysed by electrospray MS in positive ion mode. Data were summed across each of the elution regions in the TICs shown in panels a and b to generate the spectra for each class shown in the panels c-h. The y-axis shows the % intensity in counts per second (cps) normalized to 100% for the most intense peak in the spectrum across the mass range analysed. For each class of phospholipid, the y-axes of the bulk membrane and SMALP spectra are linked to allow a relative comparison of intensity. Note that phospholipid signals give m/z values with decimal 0.5 and signals with m/z values ending 0.2 or 0.3 are background contaminants. Identifications of the individual phospholipids are given in Table 1.