Table 1.

Phospholipid species detected by high resolution LC-MS/MS in SMALP-extracted P. pastoris samples.

Experimental m/z	Retention time (min)	Molecular formula	Calculated m/z	Error in ppm	Phospholipid species
Glycerophosphocholines ([MH]+)
732.5528	19.36	C40H79NO8P	732.5543	2.1	GPC (32:1)
744.5530	19.27	C41H79NO8P	744.5543	1.8	GPC (16:1/17:1)a
746.5673	19.31	C41H81NO8P	746.5700	3.6	GPC (33:1)
754.5373	19.27	C42H77NO8P	754.5387	1.8	GPC (34:4)
756.5529	19.24	C42H79NO8P	756.5543	1.9	GPC (34:3)
758.5667	19.25	C42H81NO8P	758.5694	3.7	GPC (16:1/18:1)a
760.5828	19.27	C42H83NO8P	760.5851	3.7	GPC (34:1)
768.5510	19.22	C43H79NO8P	768.5538	3.6	GPC (18:3/17:1)a
770.5667	19.20	C43H81NO8P	770.5694	3.5	GPC (35:3)
772.5844	19.20	C43H83NO8P	772.5851	1.6	GPC (18:1/17:1)a
780.5526	19.22	C44H79NO8P	780.5538	2.2	GPC (18:3/18:2)a
782.5667	19.18	C44H81NO8P	782.5695	3.6	GPC (18:2/18:2)a
784.5823	19.14	C44H83NO8P	784.5851	3.3	GPC (36:3)
786.5979	19.14	C44H85NO8P	786.6008	3.7	GPC (18:1/18:1)a
Glycerophosphoethanolamines ([MH]+)
690.5063	12.43	C37H73NO8P	690.5074	1.6	GPE 32:1)
704.5217	12.54	C38H75NO8P	704.5230	1.9	GPE (33:1)
716.5220	12.73	C39H75NO8P	716.5230	1.4	GPE (34:2)
718.5370	12.68	C39H75NO8P	718.5387	2.3	GPE (34:1)
738.5059	12.47	C41H73NO8P	738.5074	2.0	GPE (36:5)
742.5369	12.67	C41H77NO8P	742.5387	2.4	GPE (36:3)
744.5528	12.70	C41H79NO8P	744.5543	2.1	GPE (18:0/18:2)a
764.5201	12.65	C43H75NO8P	764.5230	3.8	GPE (38:6)
766.5348	12.65	C43H77NO8P	766.5387	5.1	GPE (38:5)
Glycerophosphoserines ([MH]+)
762.526	14.12	C40H77NO10P	762.5285	3.3	GPS (34:1)

For other phospholipid species the total fatty acyl chain length: double bonds are given.

^aFatty acyl chain composition determined by observation of fatty acyl ions in MS/MS spectra.