Figure 5.

CD45.2⁺CD19⁺CD21^hiCD24^hiB Cells from Butyrate-Supplemented WT but Not AhR^−/− Mice Retain Their Phenotype and Differentiate in IL-10⁺Bregs upon Adoptive Transfer

(A and B) Representative flow cytometry plots show (A) CD45.2⁺CD19⁺B cell and (B) CD45.2⁺CD19⁺CD21^hiCD24^hi B cell frequency in CD45.1 congenic WT mice that had received a transfer of CD19⁺CD21^hiCD24^hiB cells isolated from control or butyrate-supplemented WT or AhR^−/− mice.

(C) Bar chart shows number of CD45.2⁺CD19⁺CD21^hiCD24^hiB cells recovered post-transfer from CD45.1 congenic WT mice that had received a transfer of CD19⁺CD21^hiCD24^hiB cells isolated from control or butyrate-supplemented WT or AhR^−/− mice.

(D) Representative flow cytometry plots and bar charts show CD45.2⁺CD19⁺IL-10⁺B cell frequency in CD45.1 congenic WT mice that had received a transfer of CD19⁺CD21^hiCD24^hiB cells isolated from control or butyrate-supplemented WT or AhR^−/− mice.

(E) Bar chart shows number of CD45.2⁺CD19⁺IL-10⁺B cells recovered post-transfer from CD45.1 congenic WT mice that had received a transfer of control or butyrate-supplemented WT or AhR^−/− mice.

Cells were isolated at 48 h post-transfer (cumulative n = 3 per group, cumulative data are shown). Data represent mean ± SE (C and E, one-way ANOVA).