Figure 1.

Identification of Non-endothelial Stromal Cell Populations within the Omentum

(A) CD45⁻CD41⁻Ter119⁻CD31⁻PDPN^+/− non-endothelial omental stromal cells were cell-sorted and underwent scRNA-seq.

(B and C) Unsupervised clustering of non-endothelial omental stromal cells visualized with UMAP, where each dot is a single cell colored by cluster assignment (B) and hierarchical cluster tree (C).

(D) Heatmap of each cell’s (column) scaled expression of DEGs (row) expressed by a minimum of 30% of cells per cluster.

(E) Gene expression distinguishing the five clusters projected onto UMAP plots. Color scaled for each gene with highest log-normalized expression level noted.

(F–J) Violin plots of canonical omental stromal cell gene expression by cluster with highest log-normalized expression value labeled for the mesothelium (F and J), Cxcl13⁺ mesothelial cells (G), Ifit⁺ mesothelial cells (H), and Ccl11⁺Pdgfra⁺ and Matn2⁺Pdgfra⁺ fibroblasts (I).

(K) Representative gating strategy of non-endothelial omental PDPN⁺PDFGRa⁺CD200⁻ (blue), PDPN⁺PDFGRa^intCD200^int (green), and PDPN⁺PDFGRa⁻CD200⁺ (red) stromal cells and level of expression of CD44 and CD55 in these populations. Fluorescence minus one control (FMO) in gray.