REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat IgG2a anti-mouse CXCL1 antibody, clone 48415	Invitrogen	Cat# MA5-23745 RRID:AB_2609463
Rat IgG2a isotype control antibody	Invitrogen	Cat# 02-9688 RRID:AB_2532970
Armenian hamster anti-mouse TCR beta chain antibody, clone h57-597	Biolegend	Cat# 109229 RRID:AB_10933263
Donkey anti-goat IgG, polyclonal antibody	Invitrogen	Cat# A-11055 RRID:AB_2534102
Donkey anti-rabbit IgG, polyclonal antibody	Invitrogen	Cat# A-31572 RRID: AB_162543
Donkey anti-Mouse IgM-Rhodamine Red-X-AffiniPure F(ab’)2 Fragment, μ Chain Specific polyclonal antibody	Jackson ImmunoResearch	Cat# 715-296-020 RRID:AB_2340833
Goat anti-mouse Cathepsin C polyclonal antibody	R&D Systems	Cat# AF1034 RRID:AB_2245504
Goat anti-mouse CCL11/Eotaxin polyclonal antibody	R&D Systems	Cat# AF420 RRID:AB_354486
Goat anti-human ENPP2/Autotaxin polyclonal antibody	R&D Systems	Cat# AF5255 RRID:AB_2277989
Goat anti-human/mouse Myeloperoxidase/MPO polyclonal antibody	R&D Systems	Cat# AF3667 RRID:AB_2250866
Mouse anti-human CD3, clone HIT3a	Biolegend	Cat# 300308 RRID:AB_314044
Mouse anti-human CD3, clone OKT3	Biolegend	Cat# 317342 RRID:AB_2563410
Mouse anti-human CD14, clone HCD14	Biolegend	Cat# 325616 RRID:AB_830689
Mouse anti-human CD19, clone HIB19	Biolegend	Cat# 302208 RRID:AB_314238
Mouse anti-human CD19, clone HIB19	Biolegend	Cat# 302216 RRID:AB_314246
Mouse anti-human CD45, clone HI30	Biolegend	Cat# 304044 RRID:AB_2563812
Mouse anti-human CD16, clone 3G8	Biolegend	Cat# 302015 RRID:AB_314215
Mouse anti-human CD56, clone MEM-188	Biolegend	Cat# 304605 RRID:AB_314447
Mouse anti-human CD15, clone W6D3	Biolegend	Cat# 323038 RRID:AB_2564103
Rabbit anti-mouse Gro alpha/CXCL1 antibody polyclonal	Abcam	Cat# ab86436 RRID:AB_2087574
Rabbit anti-histone H3 (Citrulline 2 + 8+ 17) polyclonal antibody	Abcam	Cat# ab510 RRID:AB_304752
Rabbit anti-ISG15 polyclonal antibody	Invitrogen	Cat# PA5-17461 RRID AB_10979338
Rat anti-mouse MHC Class II (I-A/I-E) antibody, clone M5/114.15.2	Invitrogen	Cat# 47-5321-82 RRID: AB_1548783
Rat anti-mouse/human CD11b antibody, clone M1/70	Biolegend	Cat# 101256 RRID:AB_2563648
Rat anti-mouse/human CD11b antibody, clone M1/70	Biolegend	Cat# 101222 RRID:AB_493705
Rat anti-mouse CD19 antibody, clone 6D5	Biolegend	Cat# 115549 RRID:AB_2751271
Rat anti-mouse CD31 antibody, clone 390	Biolegend	Cat# 102421 RRID:AB_10613457
Rat anti-mouse CD41 antibody, clone MWReg30	Biolegend	Cat# 133927 RRID:AB_2572131
Rat anti-mouse CD44 antibody, clone IM7.8.1	Miltenyi	Cat# 130-102-606 RRID:AB_2658181
Rat anti-mouse CD45 antibody, clone 104	Biolegend	Cat# 109836 RRID:AB_2563065
Rat anti-mouse CD45 antibody, clone 104	Biolegend	Cat# 109824 RRID:AB_830789
Rat anti-mouse CD55 antibody clone REA300	Miltenyi	Cat# 130-104-026 RRID:AB_2658705
Rat anti-mouse CD200 antibody, clone OX90	Biolegend	Cat# 123807 RRID:AB_2275651
Rat anti-CXCL13 antibody, clone DS8CX13	Thermofisher	Cat# 17-7981-82 RRID:AB_2762702
Rat anti-F4/80 antibody, clone BM8	eBiosciences	Cat# 25-4801-82 RRID:AB_469653
Rat anti-Ly6C antibody, clone HK1.4	Biolegend	Cat# 128024 RRID:AB_10643270
Rat anti-Ly6G antibody, clone 1A8	Biolegend	Cat# 127610 RRID:AB_1134159
Rat anti-Ly6G antibody, clone 1A8	Biolegend	Cat# 127627 RRID:AB_10897944
Rat anti-Ly6C/Ly6G purified antibody, clone RB6-8C5	Biolegend	Cat# 108453 RRID:AB_2616681
Rat anti-mouse CD140a antibody, clone APA5	Biolegend	Cat# 135905 RRID:AB_1953268
Rat anti-mouse Siglec-F antibody, clone E50-2440	BD Biosciences	Cat# 562681 RRID:AB_2722581
Rat anti-mouse Ter119 antibody, clone TER-119	Biolegend	Cat# 116223 RRID:AB_2137788
Syrian hamster anti-mouse podoplanin, clone 8.1.1	Biolegend	Cat# 127406 RRID:AB_2161930
Syrian hamster anti-mouse podoplanin, clone 8.1.1	Biolegend	Cat# 127410 RRID:AB_10613649
Syrian hamster anti-mouse podoplanin, clone 8.1.1	Biolegend	Cat# 127412 RRID:AB_10613648
Bacterial and Virus Strains
Escherichia coli K12 bioparticles	Invitrogen	E13231
mCherry Escherichia coli	Amarh et al., 2018	N/A
Zymosan-A S. cerevisiae bioparticles fluorescein conjugate	Invitrogen	Z8241
Zymosan-A S. cerevisiae	Sigma	Z4250
Biological Samples
Human blood, omentum & peritoneal washings	Royal Infirmary of Edinburgh, NHS Lothian	2016/0035
Chemicals, Peptides, and Recombinant Proteins
4′,6-diamidino-2-phenylindole	Sigma	D9542
Collagenase I	Worthington	CLS-1
Collagenase D	Roche	11088858001
GSK484	Cayman chemicals	17488
Critical Commercial Assays
Chromium Single Cell 3′ library and gel bead kit (v2)	10X Genomics	120267
High capacity cDNA Reverse Transcription Kit	Applied Biosystems	4368814
LEGENDplex Human Inflammation Panel	Biolegend	740879
LEGENDplex Human Pro-inflammatory Chemokine	Biolegend	740003
LEGENDplex Mouse Pro-inflammatory chemokine panel	Biolegend	740451
LIVE/DEAD Fixable Blue Dead Cell Stain Kit	Invitrogen	L23105
NovaSeq 6000 S1 Reagent Kit	Illumina	20012864
RNeasy plus micro Kit	QIAGEN	74034
ToxinSensor™ Chromogenic LAL Endotoxin Assay Kit	Genscript	L00350
Triton X-100	Sigma	X100
SYTOX Blue nucleic acid stain	Invitrogen	S11384
Deposited Data
scRNaseq dataset	GEO	GSM4053741
Experimental Models: Organisms/Strains
Mouse: C57BL/6JOlaHsd	Bred in house at University of Edinburgh animal facilities	N/A
Mouse: Elane−/− (C57BL/6J)	Belaaouaj et al., 1998	N/A
Oligonucleotides
Cxcl1 Taqman gene expression assay	Thermofisher	Mm04207460_m1
Gapdh Taqman gene expression assay	Thermofisher	Mm9999995_g1
Software and Algorithms
FlowJo 10	FLOWJO, LLC	https://www.flowjo.com
Prism 7	GraphPad Software	https://www.graphpad.com/scientific-software/prism
Fiji	ImageJ	https://imagej.net/Fiji
R	The R Foundation	www.r-project.org
Scmap version 1.8.0	Kiselev et al., 2018	https://bioconductor.org/packages/release/bioc/html/scmap.html
Salmon version 0.14.1	Patro et al., 2017	https://github.com/COMBINE-lab/salmon/releases
DESeq2	Love et al., 2014	https://bioconductor.org/packages/release/bioc/html/DESeq2.html
apeglm	Zhu et al., 2019	https://bioconductor.org/packages/release/bioc/html/apeglm.html
EnhancedVolcano version 1.4.0	Blighe and Lewis, 2019	http://bioconductor.org/packages/release/bioc/html/EnhancedVolcano.html
slingshot	Street et al., 2018	https://github.com/kstreet13/slingshot
Seurat R package version 3.1.1	Stuart et al., 2019	https://github.com/satijalab/seurat/releases/tag/v3.1.1
Cell Ranger v3.0.2 Single-Cell Software Suite	10X Genomics	https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/installation
Bioreactome	Fabregat et al., 2018	https://reactome.org
Huygens 19.0 software	Scientific Volume Imaging	https://svi.nl/Huygens-Software
LAS-X-3D	Leica	https://www.leica-microsystems.com/products/microscope-software/p/leica-las-x-ls/downloads/