Figure 2.

T-cell responses at different timepoints after vaccine administration

CD4 (A) and CD8 (B) T-cell responses were measured by intracellular cytokine staining and expressed as the percentage of cells producing IL-2, IFN-γ, or both in the different treatment groups (T1–T4) and the control group (C). CD4 and CD8 T-cell responses and magnitudes of response among positive responders to any HIV PTE_g are shown 2 weeks after the month 1 (month 1·5), month 3 (month 3·5), and month 6 (month 6·5) vaccinations and 6 months after the month 6 vaccination (month 12). The proportion of positive responders for every study group is shown at the top of every panel. Horizontal lines depict median responses. Boxplots represent the distribution of responses. Circles denote positive responders. Triangles represent negative responders. T1=two doses of NYVAC vector followed by two doses of NYVAC vector and gp120 Env protein. T2=four doses of NYVAC vector and gp120 Env protein. T3=two doses of DNA vector followed by two doses of NYVAC vector and gp120 Env protein. T4=two doses of DNA vector and gp120 Env protein followed by two doses of NYVAC vector and gp120 Env protein. C=placebo groups (C1–C4), comprising matched injections. IFN=interferon. IL=interleukin. PTE_g=global potential T-cell epitope. Env=envelope.