Figure 4.

Antibody-dependent cell-mediated cytotoxicity

Responses (upper panels) and AUC net percentage of granzyme B activity (lower panels) are shown for three different antigens in the study groups. The AUC was the non-parametric area under the net percentage granzyme B activity versus log₁₀ (dilution) curve and was calculated using the trapezoidal rule. Circles show positive responses; negative responses are shown as triangles. Boxplots represent the distribution of positive responders only. Clade C gp120 was 96ZM651_D11gp120.avi/293F; clade AE gp120 was AE.A244_gDneg_gp120/293F; and clade B was MN_gp120gDneg/293F Monomer. As control, serum samples from the treated groups were incubated with target cells not coated with gp120. T1=two doses of NYVAC vector followed by two doses of NYVAC vector and gp120 Env protein. T2=four doses of NYVAC vector and gp120 Env protein. T3=two doses of DNA vector followed by two doses of NYVAC vector and gp120 Env protein. T4=two doses of DNA vector and gp120 Env protein followed by two doses of NYVAC vector and gp120 Env protein. C=placebo groups (C1–C4), comprising matched injections. AUC=area under the curve. Env=envelope.