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Sequences of sgRNAs used in this study
| Gene | Guide 1 sequence | Guide 2 sequence |
|---|---|---|
| Ctrl (Rosa26) | GAAGATGGGCGGGAGTCTTC | – |
| HIF‐1α | TCGTTAGGCCCAGTGAGAAA | CAAGATGTGAGCTCACATTG |
| P5CS (Aldh18a1) | CTTGCCGTGGGCACGACTGA | GCCGCACGCTCTGAGACAGA |
| Pycr1 | ACCCCGGTCGTGGTACGGGA | ACGGTCTCGGCCCTCCGGGT |
| Pycr2 | GCTGACCACGCGCTCACCCG | CGTGGGCAGGTCCATATCCG |
| Smad4 | AGACAGGCATCGTTACTTGT | AACTCTGTACAAAGACCGCG |
| Slc25a1 | CTTCACGTATTCGGTCGGGA | AGTGGTAGTCGTGTGCCCTA |
