Figure EV1. TG2 is beneficial for bacterial development (related to Fig 2).

• A
  HeLa cells were pre‐treated with the indicated concentrations of cysteamine for 2 h before being infected with L2^incDGFP at MOI = 0.15. Thirty hours later, the cells were disrupted and bacterial titers (IFU) were determined by re‐infecting fresh HeLa cells as described in the methods. The mean ± SD of three independent experiments is shown. P‐values of Student's paired t‐test are indicated.
• B
  TG2^+/+ and TG2^−/− MEFs were infected with L2^incDGFP at MOI = 0.15. Thirty hours later, the cells were disrupted and bacterial titers were determined by re‐infecting fresh TG2^+/+ cells as described in the methods. The mean ± SD of five independent experiments and P‐value from Student's paired t‐test are shown.
• C
  To measure bacterial adhesion, TG2^+/+ and TG2^−/− MEFs were incubated at 4°C for 4 h with L2^incDGFP at MOI = 10 before being washed and fixed as described in the methods. The mean ± SD of three independent experiments is shown.
• D
  TG2^+/+ and TG2^−/− MEFs were infected with L2^incDGFP at MOI = 10 and fixed at the indicated time. Extracellular bacteria were differentially labeled as described in the methods. The mean ± SD of three independent experiments, and P‐values from Student's paired t‐test, is shown.