Table 1.

List of primers used for real-time PCR using SYBR-Green.

Gene	Forward sequence (5′→3′)	Reverse sequence (5′→3′)
ACE (m)	CTGAACCCCATTCCCAGTCC	AATTGACGCGGTTGGACTCT
ACE2 (m)	ACACTCTGGGAATGAGGACAC	ACACTCTGGGAATGAGGACAC
AT1R (m)	ATGGCTGGCATTTTGTCTGG	GTTGAGTTGGTCTCAGACAC
Mas (m)	GGCAGGATCTATTCCCAGAAGAA	CAGTCACCCTGGAACCAAGC
CPT-1a (m)	ACTCCGCTCGCTCATTCCG	CACACCCACCACCACGATAA
ACOX-1 (m)	AGGTTGTCATCGCTTTGG	GTGATTAACTCTGGATTGAAG
G6P (m)	AGACTCCCAGGACTGGTTCA	GTCCAGGACCCACCAATACG
PEPCK (m)	ACACCAATGGGGGTTTTGGT	AAAGGTAAGGAAGGGCGGTG
IκBα (m)	CCTGACCTGGTTTCGCTCTT	AGGTAAGCTGGTAGGGGGAG
NF-κB p65 (m)	CACCAAGGATCCACCTCACC	CTCTATAGGAACTATGGATACTGCG
IL-1β (m)	TGCCACCTTTTGACAGTGATG	AAGGTCCACGGGAAAGACAC
ACE (h)	CTCTTATTGGCCAGGGGACG	AAGTCCTGCAGTAGCCCAAC
AT1R (h)	GCGCGGGTTTGATATTTGACA	TCAAATACACCTGGTGCCGA
ACE2 (h)	CATTGGAGCAAGTGTTTGGATCTT	GAGCTAATGCATGCCATTCTCA
Mas (h)	GGGAATGCACATCGGCAAAT	CTCATCCGGAAGCACAGGAA
CPT-1a (h)	TTTGGACCGGTTGCTGATGA	TTTGGACCGGTTGCTGATGA
ACOX-1 (h)	GCTGGAGCTGCGGATTTAGA	AGCTTTTCTCGGGAAAGGAGGC
G6P (h)	CACTTCCGTGCCCCTGATAA	GTAGTATACACCTGCTGTGCCC
PEPCK (h)	AGTGATGGTGGCGTGTACTG	CTGGGACTGGAAACTGCAAAC
IκBα (h)	TGTGCTTCGAGTGACTGACC	TCACCCCACATCACTGAACG
NF-κB p65 (h)	TGGCCCCTATGTGGAGATCA	AGGGGTTGTTGTTGGTCTGG
IL-1β (h)	TGAGCTCGCCAGTGAAATGAT	TCCATGGCCACAACAACTGA
18s	GGA GAA CTC ACG GAC GAC GA	CCA GTG GTC TTG GTG TGC TG

ACE2, angiotensin-converting enzyme 2; CPT-1a, carnitine palmitoyltransferase 1a; ACOX1, acyl-CoA oxidase 1; G6P, glucose-6-phosphate isomerase; PEPCK, Phosphoenol pyruvate carboxykinase; NF-κB nuclear factor κB; (m), mouse; (h), human.