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. 2020 Mar 13;8(1):127. doi: 10.3390/vaccines8010127

Figure 3.

Figure 3

Immunofluorescence analysis of human tau proteins expressed by MVA-Tau4R2N and MVA-Tau3RC. HeLa cells were infected at 0.5 PFU/cell with MVA-Tau4R2N, MVA-Tau3RC, or MVA-WT for 24 h. Then, cells were fixed and permeabilized, followed by labeling with a mouse monoclonal anti-tau antibody (7.51), or a rabbit anti-tubulin antibody. Anti-tau was detected with a mouse secondary antibody conjugated with the fluorochrome Alexa Fluor 488 (green), and anti-tubulin was detected with a rabbit secondary antibody conjugated with Alexa Fluor 594 (red). Cell nuclei were stained using DAPI (blue). Scale bar: 10 μm.