Figure 4.

Analysis of cytokine and antibody responses. (A–C) Comparison of cytokine production by antigens stimulated splenocytes from immunized and control mice. 10 days after the final immunization, spleens (n = 5) were processed and stimulated recombinant proteins, and the levels of IFN-γ, IL-5 and IL-17A in each culture supernatant were measured after 72 h. The differences were compared using Student’s t-test. (D) Comparison of serum IgG1, IgG2a and IgG2b subtypes among antigens in the immunized mice (n = 5). Serum was obtained at 10 days after the final immunization, and the levels of IgG1, IgG2a and IgG2b subtypes were expressed as the mean of log₂ titers. (E) The specific IgG of mHI_N2, MntC, mSEB and SpA5 in mice immunized with rFSAV was detected at each time point from days 0 to 42, and the levels of each antigen specific IgG were expressed as Mean Fold Increase (MFI). (D,E) Data were shown as the mean ± SEM. The p-values were calculated using one-way ANOVA (ns = no significance).