Table 4.
Histological properties in TS/A carcinoma after GET pMCAM and single-dose irradiation.
| Group | % of Proliferative Cells (AM ± SE) | % of Necrosis (AM ± SE) | % Apoptosis (AM ± SE) | % of Hypoxic Cells (AM ± SE) | Number of Blood Vessels/Field of View (AM ± SE) | % of Immune Cells (AM ± SE) |
|---|---|---|---|---|---|---|
| Control | 53.1 ± 2.7 | 11.2 ± 2.9 | 3.5 ± 0.7 | 12.3 ± 1.7 | 43.9 ± 2.5 | 3.4 ± 0.6 |
| 3× pControl | 51.4 ± 1.8 | 11.5 ± 2.5 | 4.4 ± 0.6 | 18.8 ± 1.7 | 38.9 ± 1.8 | 4.0 ± 0.7 |
| 3× pMCAM | 53.4 ± 2.1 | 13.3 ± 2.5 | 4.2 ± 0.5 | 18.6 ± 2.3 | 39.7 ± 3.2 | 3.2 ± 0.6 |
| 3× EP | 46.6 ± 2.1 | 28.8 ± 4.3 | 5.0 ± 0.6 | 22.9 ± 1.8 | 26.7 ± 1.0 | 3.3 ± 0.6 |
| 3× GETpControl | 51.2 ± 2.0 | 21.7 ± 2.1 | 12.6 ± 1.2 | 33.5 ± 2.6 | 20.5 ± 1.7 | 6.1 ± 0.8 |
| 3× GET pMCAM | 44.9 ± 1.9 | 40.0 ± 8.9 | 9.1 ± 1.1 | 33.1 ± 1.7 | 13.9 ± 1.1 | 7.3 ± 0.8 |
| IR | 33.6 ± 2.2 | 20.3 ± 3.0 | 4.2 ± 0.6 | 13.4 ± 1.8 | 27.0 ± 1.6 | 6.1 ± 0.9 |
| 3× pControl+IR | 39.5 ± 1.6 | 24.2 ± 1.5 | 6.9 ± 0.9 | 17.6 ± 1.2 | 24.7 ± 1.6 | 5.7 ± 0.8 |
| 3× pMCAM+IR | 32.9 ± 1.4 | 23.8 ± 4.0 | 6.4 ± 0.7 | 18.3 ± 0.9 | 24.6 ± 2.3 | 8.4 ± 1.1 |
| 3× EP+IR | 30.8 ± 1.5 | 34.2 ± 3.7 | 6.5 ± 0.8 | 31.8 ± 2.7 | 26.5 ± 1.6 | 7.6 ± 0.6 |
| 3× GET pControl+IR | 33.8 ± 1.3 | 53.0 ± 4.8 | 16.1 ± 1.4 | 47.0 ± 1.5 | 19.1 ± 1.2 | 8.8 ± 1.0 |
| 3× GET pMCAM+IR | 25.7 ± 2.1* | 64.2 ± 4.0** | 27.6 ± 2.9** | 54.4 ± 2.1** | 11.0 ± 1.2** | 21.9 ± 2.1** |
AM: arithmetic mean; SE: standard error of arithmetic mean; EP: application of electric pulses; GET: gene electrotransfer; IR: single-dose irradiation, 15 Gy; pMCAM: intratumorally injection of plasmid DNA-encoding shRNA for MCAM; pControl: intratumorally injection of control plasmid; * p < 0.05: statistically significant difference compared to groups: control, 3× EP, 3× pControl, 3× pMCAM; ** p < 0.05: statistically significant difference compared to groups: control, 3× EP, 3× pControl, 3× pMCAM, IR.