TABLE 1.

DMSP consumption and metabolic demands of R. pomeroyi and R. lacuscaerulensis chemostat cultures

Parameter	Valuea for:
	R. pomeroyi		R. lacuscaerulensis
Inflow DMSP	10	500	10	500
DMS produced	0.41 ± 0.03	93.6 ± 12.3	0.01 ± 0.00	25.9 ± 7.6
MeSH produced	0.70 ± 0.51	35.3 ± 4.0	1.99 ± 0.28	11.2 ± 0.9
Outflow DMSP	0.03 ± 0.03	6.06 ± 1.63	4.82 ± 1.12	362.4 ± 5.7
CH3 (DMSP) consumedb	9.56 ± 0.06	400.4 ± 12.5	5.16 ± 1.12	111.7 ± 9.2
MeSH consumedc	8.85 ± 0.47	365.0 ± 15.4	3.18 ± 1.33	100.6 ± 8.9
S demandd	3.43 ± 0.12	3.86 ± 0.13	2.85 ± 0.17	3.92 ± 0.25
C-1 demandd	15.6 ± 0.6	17.5 ± 0.6	12.9 ± 0.8	17.7 ± 1.1
Biomass Ce	425 ± 15	478 ± 16	353 ± 22	485 ± 31

^aUnless stated otherwise, all values are reported in nanomoles per minute and are the means (n = 6) of results for days 2 through 4 after the addition of either 100 μM DMSP or 5 mM DMSP to the chemostat reservoir. The error indicates the 95% confidence intervals. Abbreviations: DMSP, dimethylsulfoniopropionate; DMS, dimethylsulfide; MeSH, methanethiol; C-1, reduced C-1.

^bAmount of CH₃ C consumed by the initial demethylation of DMSP. Values were calculated by subtracting the rates of DMS production and outflow DMSP from the rate of inflow DMSP.

^cAmount of both the CH₃ C consumed by the assimilation of methanethiol by either the direct capture or reassembly pathways and the total S consumed. Values were calculated by subtracting the rates of DMS and MeSH gas production and outflow DMSP from the rate of inflow DMSP.

^dThe reduced carbon and sulfur demands were calculated from the cell yields as described in Materials and Methods.

^eThe cellular carbon was calculated as described in Materials and Methods.