TABLE 2.

DMSP consumption and metabolic demands of R. pomeroyi chemostat cultures during the labeling experiment

Parameter	Value for R. pomeroyia
Inflow DMSP	10
DMS produced	0.35 ± 0.02
MeSH produced	0.13 ± 0.04
Outflow DMSP	0.08 ± 0.01
CH3 (DMSP) consumedb	9.57 ± 0.02
MeSH consumedc	9.43 ± 0.04
S demandd	3.28 ± 0.21
C-1 demandd	13.3 ± 1.4
Biomass Ce	410 ± 20

^aUnless stated otherwise, all values are reported in nanomoles per minute and are the means (n = 10) of results for days 2 through 6 after the addition of 100 μM DMSP (50 μM unenriched DMSP and 50 μM [¹³C, ³⁴S]DMSP) to the chemostat reservoir. The error indicates the 95% confidence intervals. Abbreviations: DMSP, dimethylsulfoniopropionate; DMS, dimethylsulfide; MeSH, methanethiol; C-1, reduced C-1.

^bAmount of CH₃ consumed by the demethylation of DMSP. Values were calculated by subtracting the rates of DMS production and outflow DMSP from the rate of inflow DMSP.

^cAmount of both the CH₃ consumed by the assimilation of methanethiol by either the direct capture or reassembly pathway and the total S consumed. Values were calculated by subtracting the rates of DMS and MeSH production and outflow DMSP from the rate of inflow DMSP.

^dThe reduced carbon and sulfur demands were calculated from the cell yields as described in Materials and Methods.

^eValues are the means (n = 5) of results for days 2 through 6 after the addition of 100 μM DMSP (50 μM unenriched DMSP and 50 μM [¹³C, ³⁴S]DMSP) to the chemostat reservoir. The cellular carbon was calculated as described in Materials and Methods.