FIG 1.

Energy-dependent azole efflux inhibition with clorgyline. Intracellular [³H]FLC concentration was measured in cells that were treated with [³H]FLC alone (19.25 nM), [³H]FLC with 2% glucose (efflux active), [³H]FLC with 2% glucose and clorgyline (233 μM), and with [³H]FLC with clorgyline. Heat killed (HK) represents the nonspecific, background-level [³H]FLC accumulation as the cells have been killed at 95°C for 10 min. [³H]FLC accumulation was measured after 20 h under all conditions. Error bars represent the standard deviation of biological triplicates for each condition.