TABLE 1.
Strains and plasmids used in this work
| Plasmid or strain | Genotype and/or descriptiona | Reference or source |
|---|---|---|
| Plasmids | ||
| pTA131 | pBluescript II with BamHI-XbaI fragments from pGB70 harboring p.fdx-pyrE2, Ampr | 19 |
| pTA963 | pyrE2 and hdrB markers, Trp-inducible (p.tna) promoter, Ampr | 43 |
| pFH25 | pTA963 carrying hvo_0405 with C-terminal 6×His tag | 12 |
| pFH38 | pTA131 carrying 700 bp upstream and 700 bp downstream hvpssA flanking region | This work |
| pFH43 | pTA131 carrying 700 bp upstream and 700 bp downstream hvpssD flanking region | This work |
| pFH39 | pTA963 carrying hvpssA with C-terminal 6×His tag | This work |
| pFH44 | pTA963 carrying hvpssD with C-terminal 6×His tag | This work |
| pFH55 | pTA963 carrying hvo_0405 N-terminal LVIVD domain with C-terminal 6×His tag | This work |
| Strains | ||
| DH5α | E. coli F− ϕ80ΔlacZΔM15 (lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) phoA supE44 thi-1 gyrA96 relA1 | Invitrogen |
| DL739 | E. coli MC4100 recA dam-13::Tn9 | 48 |
| H26 | H. volcanii ΔpyrE2 | 19 |
| H53 | H. volcanii ΔpyrE2 ΔtrpA | 19 |
| FH27 | H53/pTA963::hvo_0405-6×His | 12 |
| FH28 | H53 ΔartA/pTA963::hvo_0405-6×His | 12 |
| FH55 | H53 ΔhvpssA/pTA963 | This work |
| FH56 | H53 ΔhvpssA/pTA963::hvpssA-6×His | This work |
| FH57 | H53 ΔhvpssA/pTA963::hvo_0405-6×His | This work |
| FH69 | H53 ΔhvpssD/pTA963 | This work |
| FH70 | H53 ΔhvpssD/pTA963::hvpssD-6×His | This work |
| FH71 | H53 ΔhvpssD/pTA963::hvo_0405-6×His | This work |
| FH77 | H53 Δhvo_0405/pTA963::N-term-hvo_0405-6×His | This work |
| aBL128 | ΔpyrE2 artA::artA-msfGFP-pyrE2 | This work |
| aBL129 | ΔpyrE2 hvpssD::hvpssD-msfGFP-pyrE2 | This work |
| aBL183 | ΔpyrE2 hvpssA::hvpssA-msfGFP-pyrE2 | This work |
| aBL131 | ΔpyrE2 ftsZ1::ftsZ1-msfGFP-pyrE2 | This work |
| aBL184 | ΔpyrE2/pTA962::msfGFP(SW) | This work |
| aBL118 | ΔpyrE2/pTA962::csg-msfGFP(SW) | This work |
a
Ampr, ampicillin resistant.