FIG 5.

Phage genome segments that support autonomous replication. Segments of eight phage genome maps are shown with relevant genes labeled; the locations of parS repeats are indicated by black boxes on the genome ruler. A black arrow indicates the location and transcription direction of the noncoding RNA implicated in replication initiation. Bars underneath each map indicate a genome segment inserted into the nonreplicating vector pMOS-Hyg and then electroporated into M. smegmatis mc²155. Replication-proficient plasmids efficiently transforming M. smegmatis (>10⁴ CFU/μg DNA) are shown in green, and those that fail to transform are shown in red. A black X indicates the position of a stop codon introduced by mutagenesis.