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Maintenance of plasmids without selection. M. smegmatis transformants with plasmids (as indicated) were grown in liquid culture with selection to saturation and then serially passaged for a total of ∼40 generations without selection. The percentage of plasmid maintenance was determined by plating serial dilutions of culture on solid media with and without selection. Data represent the mean values from four independent cultures, and error bars represent one standard deviation.
