TABLE 3.
Transformation efficiencies of phage-based plasmids in M. smegmatis
| Plasmid | Phagea | Feature(s) | Transformation efficiency (CFU/μg of DNA) |
|---|---|---|---|
| pCCK38 | N/A | oriM | 1.2 × 105 |
| pHA06 | Et2Brutus | ori + parABS | 1.1 × 105 |
| pHA08 | Et2Brutus | ori | 3.2 × 104 |
| pKZ05 | Gladiator | ori + parABS | 9.6 × 104 |
| pKZ07 | Gladiator | ori | 1.2 × 105 |
| pHA01 | LadyBird | ori + parABS | 6.5 × 104 |
| pHA03 | LadyBird | ori | 1.2 × 105 |
| pKZ01 | Alma | ori + parABS | 1.0 × 105 |
| pKZ02 | Alma | ori | 1.3 × 105 |
| pKSW39 | RedRock | ori + parABS | 8.9 × 104 |
| pKSW60 | RedRock | ori | 6.6 × 104 |
| pKSW07 | Miko | repA + parABS | 9.0 × 104 |
| pKSW09 | Miko | repA | 1.0 × 105 |
| pKSW08 | Rachaly | repA + parABS | 9.7 × 104 |
| pKSW10 | Rachaly | repA | 8.1 × 104 |
| pKSW50 | Jeeves | repA + parABS | 1.1 × 105 |
| pKSW52 | Jeeves | repA | 8.4 × 104 |
| pMOS-Hyg | N/A | None | 0 |
a
N/A, not applicable.