FIG 4.

Discovery of a polytheonamide-like RiPP biosynthetic gene cluster. (A) Comparison of the polytheonamide (Poy) biosynthetic BGC and a BGC from the genome of “Candidatus Caria hoplita” (Ch) that is predicted to encode a related compound. Genes are colored by predicted function. The inset orange box indicates regions of conservation and divergence between the predicted precursor peptides found in each pathway. (B) Predicted structure for the final product of the novel M. hentscheli RiPP cluster. All possible methylations are shown in red; however, it is likely that only a subset of these occur in the final compound.