FIG 9.

Scheme of MX1 and LGALS8 regulation by miRNA precursor pool transfection to influence L. pneumophila replication in macrophages. Precursor transfection of miR-125b reduces the expression of TP53, while DDX58 (RIG-I) is targeted by miR-221. Both targets further regulate the protein expression of MX1, which has an antibacterial effect on Legionella. Furthermore, miR-579 acts on LGALS8 to reduce its expression. Thus, reduction of MX1 and LGALS8 expression leads to an increased replication of Legionella in macrophages. In conclusion, these miRNAs are influencing the expression of their targets and have an impact on Legionella replication.