Figure 1.

Generation and analysis of an ADSL deletion mutant. (a) Schematic illustration of knocking out ADSL in ME49 to produce ΔADSL. (b) Diagnostic polymerase chain reaction (PCR) for a ΔADSL mutant clone. (c, d) Plaque assay comparing the growth of the ΔADSL mutant to that of the wild-type strain ME49. (e) Tachyzoite replication under standard tissue culture conditions. HFF cells were infected with purified tachyzoites, invading parasites were cultured for 24 h, and then fluorescently stained to determine the number of parasites in each parasitophorous vacuole (PV), ** p < 0.01, two-way analysis of variance.