Table 1.

Primers used in this study.

Primer	Sequence	Use
gRNA-ADSL-Fw	5′- GACACTGCTCAATTTCGTCGGTTTTAGAGCTAGAAATAGC-3′	To construct the ADSL specific CRISPR plasmid
gRNA-R	5′-AACTTGACAT CCCCA TTTAC-3′	To construct the ADSL specific CRISPR plasmid
pUC19-Fw pUC19-Rv	5′-GGCGTAATCATGGTCATAGC-3′ 5′-CTCGAATTCACTGGCCGTCG-3′	Amplification of pUC19 for Gibson assembly Amplification of pUC19 for Gibson assembly
loxp-DHFR*-loxp-Fw loxp-DHFR*-loxp-Rv 5′-GGACACGCTGAACTTGTGGC-3′	5′-CAACCCGCGCAGAAGACATC-3′	Amplification of loxp-DHFR*-loxp for Gibson assembly Amplification of loxp-DHFR*-loxp for Gibson assembly
U5ADSL-Fw pADSL: loxp- DHFR*-loxp construction	5′-GTTGTAAAACGACGGCCAGTCTAATTTTTGCCGGGTCTGG-3′	Amplification of 5′-homology of ADSL for
U5ADSL-Rv pADSL: loxp- DHFR*-loxp construction	5′-GATGTCTTCTGCGCGGGTTG CGAGACGAAGAAGAGAGC-3′	Amplification of 5′-homology of ADSL for
U3ADSL-Fw pADSL: loxp -DHFR*-loxp construction	5′-GCCACAAGTTCAGCGTGTCCACCTCAGTTGTCGGCACCGT-3′	Amplification of 3′-homology of ADSL for
U3ADSL-Rv pADSL: loxp--DHFR*-loxp construction	5′-GCTATGACCATGATTACGCCCGCAGACTCAAATCTATTC-3′	Amplification of 3′-homology of ADSL for
5′-UpU5 ADSL	5′-GCTCTTGCTTTCGTCGCTGTC-3′	PCR1 of ΔADSL:DHFR
3′-InDHFR*-Fw	5′-CGTGACCACGCCAAAGTAG-3′	PCR1 of ΔADSL:DHFR
5′-InDHFR*-Rv	5′-GCACTTGCAGGATGAATTCC-3′	PCR2 of ΔADSL:DHFR
3′-DnU3ADSL	5′-GTGTCTCGCACATGCGCGTT-3′	PCR2 of ΔADSL:DHFR
5′-UpgRNA ADSL	5′- AACGCAATCAAGACGCTGGC-3′	PCR3 of ΔADSL:DHFR
3′-DngRNA ADSL	5′- CGTCACTGTACCAGCGAGCAG-3′	PCR3 of ΔADSL:DHFR