Figure 2.

Stability of the HSV-2 US6 gene in MVA deletion (del) II and del III sites. Recombinant MVAdel2-gD2 and MVAdel3-gD2 were passaged 35 times in DF-1 cells and tested for glycoprotein D2 (gD2) expression by Western blotting and immunostaining at passages (P) 1, 5, 10, 15, 20, 25, 30, and 35, along with MVA-infected cell controls. (A) gD2 and vaccinia A27 protein expression by MVAdel2-gD2 evaluated by Western blotting and (B) by immunostaining. (C) gD2 and vaccinia A27 protein expression by MVAdel3-gD2 assessed by Western blotting and (D) by immunostaining. The SE₅₀ is the estimated number of passages of recombinant MVA at which 50% of plaques stained positive for gD2 expression with a rabbit anti-HSV-2 polyclonal antibody. SE₅₀ was determined as the x-axis coordinate corresponding to a y-axis coordinate of 50%.