Table 1.
Recombinant modified vaccinia virus Ankara (MVA) constructs expressing HSV-2 glycoprotein D2 (gD2).
| Name | US6 Insertion Site | Plasmid Shuttle Vector | Vaccinia Promoter | US6 Gene Orientation | Reporter Gene/Orientation | Reference |
|---|---|---|---|---|---|---|
| MVAdel2-gD2 | del II | pLW22-SMN | Synthetic early/late | RRF | lacZ/RRF | [24] |
| MVAdel3-gD2 | del III | pLW44attR * | mH5 | RRF | GFP/RRF | [26] |
| MVA17-gD2 | CP77 | pCAM17 * | mH5 | RRF | GFP/RRF | - |
| MVA17-gD2rev | CP77 | pCAM17R * | mH5 | LRF | GFP/LRF | - |
| MVA18-gD2 | I8R-G1L | pCAM18 * | mH5 | RRF | GFP/RRF | - |
| MVA18-gD2rev | I8R-G1L | pCAM18R * | mH5 | LRF | GFP/LRF | - |
* Destination vector contains Reading Frame B of the Gateway Vector Conversion System (ThermoFisher, Carlsbad, CA, USA); mH5, modified vaccinia H5 promoter [21]; RRF, Rightward-Reading Frame; LRF, Leftward-Reading Frame.