Fig. 2.

Single cell transcriptome analysis of Gsx2⁺ and Ascl1⁺ progenitors show progressive maturation of LGE progenitors. (A) UMAP plot of distinct cell types identified from E12.5 ventral telencephalon cells. Clusters with characteristics of VZ and SVZ progenitors (i.e. clusters 0 and 2, respectively) are outlined and labeled. (B-D) Feature plots showing cells expressing the VZ marker Slc1a3 (Glast) (B), the SVZ-enriched Dlx1 (C) and the neuronal marker Dcx (D). (E-G) Feature plots showing Gsx2⁺ only (E), Gsx2⁺Ascl1⁺ (F) and Ascl1⁺ only (G) LGE cells within the progenitor compartments. Note that Gsx2⁺-only cells correlate well with those expressing the VZ radial glial marker Slc1a3 (Glast) (B,E), whereas the double-labeled cells and Ascl1⁺-only cells correlate best with the SVZ marker Dlx1 (C,F,G). Moreover, the Ascl1⁺-only cells correlate best with the neurogenic (i.e. Dcx⁺) cells (D,G).