Fig. 7.

Gsx2 and Tcf3 compete for molecular interactions with Ascl1 in a yeast three-hybrid assay. The yeast three-hybrid assay utilizes an interfering protein that is capable of interacting with the bait or the prey to disrupt their interaction. (A) Using Gsx2 as bait and Ascl1 as prey, with no interfering protein, results in reporter (α-gal) expression, whereas the addition of Tcf3 (E-protein) as an interfering protein disrupts the interaction as shown in B. (C) Likewise, with Tcf3 as bait and Ascl1 as prey, and no interfering protein, α-gal expression is activated, which is disrupted by the addition of Gsx2 as the interfering protein as seen in D.