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. 2020 Mar 9;9(3):200. doi: 10.3390/pathogens9030200

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression patterns of inflammatory cytokines and chemokines genes in bovine mammary epithelial (BME) cells after lipopolysaccharide (LPS) and Pam3CSK4 stimulation. The expression of interleukin 1 alpha (IL1α), IL1β, C-C motif chemokine ligand 2 (CCL2), C-X-C motif chemokine ligand 2 (CXCL2), CXCL3 and CXCL8 were determined in BME cells after 3, 6, 12 and 24 hours of LPS and Pam3CSK4 stimulation by qPCR. BME cells with no LPS treatment were used as basal controls (hour 0). The results represent data (mean ± SD) from three independent experiments. Significantly different compared to basal control: *, p < 0.05, **p < 0.01.