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. 2020 Mar 2;8(1):116. doi: 10.3390/vaccines8010116

Figure 4.

Figure 4

IAPP physiological function is maintained in the presence of anti-IAPP antibodies. (a) C57BL/6 male mice were immunized with the Qβ-Nterm (s-s) vaccine (n = 16) or with the uncoupled Qβ-VLPs (n = 14) at day 0 (week 6), day 14 (week 8) and day 28 (week 10), serum was collected at day 14 and day 34 (b) to check antibody titer. On days 36, 38, 41 and 43, mice were fasted during the light phase period for 12 h. Mice then received either IAPP or the control saline solution immediately prior to dark onset and food intake was measured 1, 2, 4 and 22 h after injection. (b) Serum antibodies were analyzed for recognition of the N-term (s-s) peptide coupled to RNase (RNase-N-term(s-s) in red), hIAPP (in peach) and rIAPP (in black) for the Qβ-Nterm (s-s) (left panel) and the Qβ-VLP (right panel)-immunized mice. (c) Cumulative food intake after 1-h refeeding and (d) baseline correction for the IAPP versus saline group in Qβ and Qβ-Nterm (s-s)-immunized mice. Additional time points can be seen in Supplementary Figure S2. Data are the means ± SEM. RNase-N-term (s-s), N-term (s-s) peptide coupled to RNase; rIAPP, rat IAPP; hIAPP, human IAPP. Statistical test in (c,d): 2-way ANOVA and Sidak’s multiple comparison. No significant difference was observed in (c,d) (p > 0.05).