Table 2.
Titration of human anti-poliovirus sera type 1, 2 and 3 with multiplex PCR-based neutralization (MPBN) and conventional neutralization assays.
| Virus Challenge | PCR Repeats | Sera Titers | ||
|---|---|---|---|---|
| Type 1 | Type 2 | Type 3 | ||
| MPBN Assay | ||||
| Sabin 1, 2 and 3 | 1 | 14,596 | 5793 | 7298 |
| 2 | 14,596 | 5793 | 7298 | |
| 3 | 14,596 | 5793 | 5793 | |
| Sabin 1 and 2 | 1 | 9195 | 5793 | |
| 2 | 9195 | 5793 | ||
| 3 | 9195 | 5793 | ||
| Sabin 2 and 3 | 1 | 5793 | 7298 | |
| 2 | 5793 | 7298 | ||
| 3 | 5793 | 7298 | ||
| Sabin 1 and 3 | 1 | 11,585 | 7298 | |
| 2 | 11,585 | 7298 | ||
| 3 | 11,585 | 5793 | ||
| Sabin 1 | 1 | 11,585 | ||
| 2 | 11,585 | |||
| 3 | 11,585 | |||
| Sabin 2 | 1 | 5793 | ||
| 2 | 5793 | |||
| 3 | 5793 | |||
| Sabin 3 | 1 | 5793 | ||
| 2 | 5793 | |||
| 3 | 5793 | |||
| Conventional Neutralization Assay | ||||
| Sabin 1, 2 and 3 | NA | 11,585 | 5793 | 13,777 |
| Mean | 11,729 | 5793 | 7218 | |
| STDEV | 1916 | 0 | 2106 | |
Note: STDEV; Standard Deviation.