Figure 2.

Cellular uptake of nanovaccine by macrophage in vitro. (A) Mean fluorescence intensity of cell uptake capability. Data are presented as the mean ± SD. p values were calculated by Student’s t-test (** p < 0.01, * p < 0.05). (B) Representative confocal microscopic images of macrophage incubated with VP7, CNTs-VP7, and CNTs-M-VP7, respectively. The unhandled macrophages served as the control. Vaccines were labeled with FITC (green channel), respectively; The cell nucleus was labeled with DAPI (blue channel); The mannose receptor was labeled with Cy3 (red channel).