Figure 5.

In vivo and ex vivo fluorescence images in vaccinated fish. (A) Representative in vivo fluorescence images for grass carp taken at different time points after vaccination; (B) Quantitative fluorescence signals of vaccinated fish; (C) Representative ex vivo fluorescence images of isolated fish tissues at five different time points; (D) Quantitative fluorescence signals of different fish tissues. Different colors in (A) and (C) represent the fluorescence intensity of FITC-CNTs-M-VP7 nanovaccine. The fluorescence intensity increases successively from purple to red. Data are means for three assays and represented as mean ± SD. p values were calculated by Duncan’s test. Data at the same sampling time with different letters (a, b, c, and d) are significantly different (p < 0.05).