Table 2.
Alfalfa samples used to detect and characterize RNA viruses.
| Sample No | Location | Symptom | Detected RNA Viruses and Detection Methods | ||||
|---|---|---|---|---|---|---|---|
| AMV | BLRV | MsAV1 * | MsAPV1 * | ARaV * | |||
| 1 | Victoria | Yellow mosaic | A, B, C | - | B | B | - |
| 2 | Victoria | Ringspot, yellow mosaic | A, B, C | - | B, C | B, C | B, C |
| 3 | South Australia | Ringspot | B, C | - | B | B | B, C |
| 4 | Victoria | Yellow patches, mild ringspot | B, C | - | B | B | - |
| 5 | Victoria | Ringspot | A, B, C | - | B | B, C | B |
| 6 | South Australia | Ringspot | B, C | - | B, C | B | - |
| 7 | Victoria | Reddening | A, B, C | - | B | B, C | - |
| 8 | Victoria | Leaf rolling, enation | B, C | - | B | B | - |
| 9 | South Australia | Ringspot, yellow patches | B, C | - | B, C | B, C | B, C |
| 10 | South Australia | Leaf curling | A, B, C | A, B, C | B | B, C | - |
Symptoms are shown in Figure 2. Viruses were detected by A: duplex RT-PCR [45], B: high throughput sequencing (HTS), and C: RT-PCR. (-): not detected. (*) viruses were first identified by HTS and then validated by RT-PCR. AMV: alfalfa mosaic virus, BLRV: bean leaf roll virus, ARaV: alfalfa ringspot-associated virus, MsAV1: Medicago sativa amalgavirus 1, MsAPV1: Medicago sativa alphapartitivirus 1.