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. 2020 Mar 18;9(3):223. doi: 10.3390/pathogens9030223

Table 2.

List of fungal pathogens on bast fiber crops identified using molecular method.

Pathogen Disease Method Marker Host Plant Geographic Region(s) Reference
Alternaria
A. alternata Hemp leaf spot Conventional PCR ITS Cannabis sativa Shanxi, China [46]
A. alternata Ramie black leaf spot Conventional PCR ITS, GAPDH Boehmeria nivea Hunan, Hubei, China [47]
Cercospora
Cercospora cf. flagellaris Hemp leaf spot disease Not mentioned ITS, EF-1α, CAL, H3, actin Cannabis sativa Kentucky, USA [48]
Colletotrichum
C. corchorum capsularis Jute anthracnose Conventional PCR ACT, TUB2, CAL, GAPDH, GS, and ITS Corchorus capsularis L. Zhejiang, Fujian, Guangxi, and Henan, China [27]
C. fructicola Jute anthracnose Conventional PCR ACT, TUB2, CAL, GAPDH, GS, and ITS Corchorus capsularis L. Zhejiang, Fujian, Guangxi, and Henan, China [26]
C. fructicola Jute anthracnose Conventional PCR ACT, TUB2, CAL, GAPDH, GS, and ITS Corchorus capsularis L. Zhejiang, Fujian, Guangxi, and Henan, China [27]
C. gloeosporioides Ramie anthracnose Conventional PCR ITS Boehmeria nivea HuBei, HuNan, JiangXi, and SiChuan, China [30]
C. higginsianum Ramie anthracnose Conventional PCR ITS Boehmeria nivea HuBei, China [29]
C. phormii New Zealand flax anthracnose Conventional PCR ITS Phormium tenax California, USA [24]
C. phormii New Zealand flax anthracnose Conventional PCR ITS Phormium tenax Perth, Australia [25]
C. siamense Jute anthracnose Conventional PCR ACT, TUB2, CAL, GAPDH, GS, and ITS Corchorus capsularis L. Zhejiang, Fujian, Guangxi, and Henan, China [26]
Colletotrichum sp. Kenaf anthracnose Conventional PCR ITS Corchorus olitorius South Korea [28]
Curvularia
C. cymbopogonis Hemp leaf spot Conventional PCR 25S Cannabis sativa USA [52]
Exserohilum
E. rostratum Hemp floral blight Not mentioned ITS, RPB2 Cannabis sativa North Carolina, USA [49]
Fusarium
F. oxysporum Hemp roots and crown rot Conventional PCR ITS, EF-1α Cannabis sativa Canada [32]
F. oxysporum Jute brown wilt Conventional PCR ITS Corchorus olitorius Dhaka, Manikgonj, Kishorgonj, Rangpur, and Monirampur, Bangladesh [40]
F. oxysporum Hemp wilt Conventional PCR ITS, EF-1α Cannabis sativa California, USA [34]
F. solani Hemp crown root Conventional PCR ITS, EF-1α Cannabis sativa Canada [32]
F. solani Hemp wilt Conventional PCR ITS, EF-1α Cannabis sativa California, USA [34]
F. solani Sunn hemp root rot and wilt Conventional PCR ITS, EF-1α Crotalaria juncea Ceará, Brazil [41]
F. brachygibbosum Hemp wilt Conventional PCR ITS, EF-1α Cannabis sativa California, USA [34]
F. udum f. sp. crotalariae Sunn hemp fusarium wilt Conventional PCR EF-1α, β-tubulin Crotalaria juncea Tainan, China [42]
Glomus
G. mosseae Hemp root rot Conventional PCR 25S Cannabis sativa USA [52]
Golovinomyces
G. spadiceus Hemp powdery mildew Not mentioned ITS, 28S Cannabis sativa Kentucky, USA [43]
G. cichoracearum sensu lato Hemp powdery mildew Conventional PCR ITS Cannabis sativa Atlantic Canada and British Columbia. [44]
G. cichoracearum Sunn hemp powdery mildew Not mentioned ITS Crotalaria juncea Florida, USA [45]
Lasiodiplodia
L. theobromae Kenaf black rot Conventional PCR ITS Corchorus olitorius Kangar Perlis, Malaysia [54]
Leptoxyphium
L. kurandae Kenaf sooty mould Conventional PCR ITS Corchorus olitorius Iksan, Korea [55]
Macrophomina
Macrophomina phaseolina Hemp charcoal rot Conventional PCR EF-1α, CAL Cannabis sativa Southern Spain [50]
Micropeltopsis
Micropeltopsis cannabis Unknown Conventional PCR 25S Cannabis sativa USA [52]
Orbilia
Orbilia luteola Unknown Conventional PCR 25S Cannabis sativa USA [52]
Pestalotiopsis
Pestalotiopsissp. Hemp spot blight Conventional PCR 25S Cannabis sativa USA [52]
Podosphaera
P. xanthii Ramie powdery mildew Conventional PCR ITS Boehmeria nivea Naju, Korea [53]
Pythium
P. dissotocum Browning and a reduction in root mass, stunting Conventional PCR ITS, EF-1α Cannabis sativa Canada [32]
P. myriotylum Browning and a reduction in root mass, stunting Conventional PCR ITS, EF-1α Cannabis sativa Canada [32]
P. myriotylum Hemp root rot and Wilt Conventional PCR ITS, COI, COII Cannabis sativa Connecticut, USA [33]
P. aphanidermatum Hemp root rot and crown wilt Conventional PCR ITS Cannabis sativa California, USA [34]
P. aphanidermatum Hemp crown and root Rot Conventional PCR ITS Cannabis sativa Indiana, USA [35]
P. ultimum Hemp crown and root Rot Conventional PCR ITS Cannabis sativa Indiana, USA [36]
Rhizoctonia
Binucleate R. spp. Hemp wilt Conventional PCR 25S Cannabis sativa USA [52]
Sclerotinia
Sclerotinia minor Hemp crown rot Conventional PCR ITS Cannabis sativa San Benito County, Canada [51]
Sphaerotheca
S. macularis Hemp powdery mildew Conventional PCR 25S Cannabis sativa USA [52]
Verticillium
V. dahliae flax wilt Conventional PCR ITS Linum usitatissimum La Haye Aubrée, France [37]
V. dahliae flax wilt qPCR ITS Linum usitatissimum Normandy, France [38]
V. dahliae flax wilt qPCR ß-tubulin Linum usitatissimum Germany [39]
V. tricorpus flax wilt qPCR ITS Linum usitatissimum Germany [39]
V. longisporum flax wilt qPCR ß-tubuIin Linum usitatissimum Germany [39]

qPCR: quantitative PCR, ITS: internal transcribed spacer, GAPDH: glyceraldehydes-3-phosphate dehydrogenase, GS: glutamate synthetase, EF-1α: translation elongation factor 1-α, CAL: calmodulin, H3: histone subunit 3, ACT: actin, TUB2: ß-tubulin, RPB2: RNA polymerase subunit B2, COI: cytochrome oxidase subunit I, COII: cytochrome oxidase subunit II.