. 2020 Mar 18;9(3):223. doi: 10.3390/pathogens9030223

Table 3.

Genes and PCR primers used for their amplification in fungal pathogens infecting bast fiber crops.

Target DNA	Primer Name and Sequence (5′-3′)		Size of PCR Product (bp)	Reference
18S	NS3	GCAAGTCTGGTGCCAGCAGCC	Not mentioned	[31]
18S	NS4	CTTCCGTCAATTCCTTTAAG	Not mentioned	[31]
28S	LR0R	GCAAGTCTGGTGCCAGCAGCC	Not mentioned	[31]
28S	LR3	GCAAGTCTGGTGCCAGCAGCC	Not mentioned	[31]
25S	LROR	ACCCGCTGAACTTAAGC	1431	[52]
25S	LR7	TACTACCACCAAGATCT	1431	[52]
ACT	ACT-512F	ATGTGCAAGGCCGGTTTCGC	300	[48]
ACT	ACT-783R	TACGAGTCCTTCTGGCCCAT	300	[48]
ß-tubulin	Vd-btub-1F	GCGACCTTAACCACCTCGTT	Not mentioned	[38]
	Vd-btub-1R	CGCGGCTGGTCAGAGGA	Not mentioned	[38]
	VertBt-F	AACAACAGTCCGATGGATAATTC	Not mentioned	[38]
	VertBt-R	GTACCGGGCTCGAGATCG	Not mentioned	[38]
	VITubF2	GCAAAACCCTACCGGGTTATG	143	[39]
	VITubRl	AGATATCCATCGGACTGTTCGTA	143	[39]
	VdTubF2	GGCCAGTGCGTAAGTTATTCT	82	[39]
	VdTubR4	ATCTGGTTACCCTGTTCATCC	82	[39]
	Bt2a	GGTAACCAAATCGGTGCTGCTTTC	Not mentioned	[26]
	Bt2b	ACCCTCAGTGTAGTGACCCTTGGC	Not mentioned	[26]
CAL	CL1	GARTWCAAGGAGGCCTTCTC	Not mentioned	[26]
	CL2	TTTTTGCATCATGAGTTGGAC	Not mentioned	[26]
	CAL-228F	GAGTTCAAGGAGGCCTTCTCCC	Not mentioned	[50]
	CAL-737R	CATCTTTCTGGCCATCATGG	Not mentioned	[50]
EF-1α	EF-1	ATGGGTAAGGAGGACAAGAC	700	[34]
	EF-2	GGAGGTACCAGTGATCATGTT	700	[34]
	EF1-728F	CATCGAGAAGTTCGAGAAGG	Not mentioned	[50]
	EF2	GGAGGTACCAGTGATCATGTT	Not mentioned	[50]
	EF1-728F	CATCGAGAAGTTCGAGAAGG	350	[48]
	EF1-983R	TACTTGAAGGAACCCTTACC	350	[48]
Endochitinase	Vd-endoch-1F	CTCGGAGGTGCCATGTACTG	Not mentioned	[38]
Endochitinase	Vd-endoch-1R	ACTGCCTGGCCCAGGTTC	Not mentioned	[38]
GAPDH	Vd-G3PD-2F	CACGGCGTCTTCAAGGGT	Not mentioned	[38]
	Vd-G3PD-1R	CAGTGGACTCGACGACGTAC	Not mentioned	[38]
	GDF1	GCCGTCAACGACCCCTTCATTGA	Not mentioned	[26]
	GDR1	GGGTGGAGTCGTACTTGAGCATGT	Not mentioned	[26]
	gpd-1	CAACGGCTTCGGTCGCATTG	Not mentioned	[47]
	gpd-2	GCCAAGCAGTTGGTTGTGC	Not mentioned	[47]
GS	GSF1	ATGGCCGAGTACATCTGG	Not mentioned	[26]
GS	GSR1	GAACCGTCGAAGTTCCAC	Not mentioned	[26]
ITS	ITS1	TCCGTAGGTGAACCTGCGG	334-738	[24,30,35,36,37,38]
	ITS4	TCCTCCGCTTATTGATATGC	334-738	[24,30,35,36,37,38]
	Vd-ITS1-45-F	CCGGTCCATCAGTCTCTCTG	334	[37]
	Vd-ITS2-379-R	ACTCCGATGCGAGCTGTAAC	334	[37]
	ITS1-F	CTTGGTCATTTAGAGGAAGTAA	700	[34]
	ITS4	TCCTCCGCTTATTGATATGC	700	[34]
	VtF4	CCGGTGTTGGGGATCTACT	123	[39]
	VtR2	GTAGGGGGTTTAGAGGCTG	123	[39]
	ITS 4	TCCTCCGCTTATTGATATGC	Not mentioned	[26]
	ITS 5	GGAAGTAAAAGTCGTAACAAGG	Not mentioned	[26]
RPB2	bRPB2-6F	TGGGGYATGGTNTGYCCYGC	Not mentioned	[49]
RPB2	bRPB2-7R	GAYTGRTTRTGRTCRGGGAAVGG	Not mentioned	[49]